Determination of L-Cysteine Based on Energy Transfer between Cu2-xSe Nanoparticles and Rhodamine B

作者：Wang Xue; Yang Kun-Cheng; Mao Zhi-Yuan; Huang Cheng-Zhi^*; Wang Jian^*

来源：Chinese Journal of Analytical Chemistry, 2016, 44(10): 1482-1486.

DOI：10.1016/S1872-2040(16)60960-X

• Cu2-xSe nanoparticles
• L-cysteine
• Photo-induced electron transfer

The fluorescence of Rhodamine B (RhB) could be quenched by the manner of photo-induced electron transfer with Cu2-xSe nanoparticles as the energy receptor and RhB as the energy donor. However, L-cysteine (L-Cys) was capable of recovering the fluorescence of Rhodamine B, and the recovered fluorescence intensity was proportional to the concentrations of L-Cys. Based on that, a novel method for detecting L-Cys was established. After mixing L-Cys and RhB pretreated by Cu2-xSe nanoparticles at pH 4.6 and 30 degrees C for 2 min, a linear relationship was obtained between the fluorescence intensity of RhB at 575 nm and the concentrations of L-Cys in the range of 2.5 x 10(-7)-1.1 x 10(-6) M. This method was used for the determination of L-cys with a detection limit (3 sigma/k) of 5.5 x 10(-8) M. The common amino acids presented little interference for the detection of L-Cys.

• 出版日期2016-10
• 单位西南大学