Toll-like receptor 9 agonists up-regulates the expression of cyclooxygenase-2 via activation of NF-κB in prostate cancer cells

作者：Di, Jin Ming; Pang, Jun; Sun, Qi Peng; Zhang, Yan; Fang, You Qiang; Liu, Xiao Pen; Zhou, Jian Hua; Ruan, Xing Xing; Gao, Xin^*

来源：Molecular Biology Reports, 2010, 37(4): 1849-1855.

DOI：10.1007/s11033-009-9620-5

• Prostate cancer
• Toll-like receptor 9
• Cyclooxygenase-2
• NF-kappa B
• CpG-oligonucleotides

CpG-oligonucleotides (CpG-ODNs), mimicking bacterial DNA, have recently been shown to stimulate prostate cancer invasion in vitro via Toll-like receptor 9 (TLR9). Since cyclooxygenase 2 (COX-2), frequently overexpressed in multiple tumor types including prostate cancer, is a causal factor for tumor development, invasion and metastasis, an interesting question is raised whether TLR9 regulates COX-2 expression in prostate cancer cells. To address this question, herein we examined COX-2 expression in PC-3 cells stimulated with different doses and time courses of CpG-ODNs. The regulatory role of NF-kappa B in TLR9-mediated COX-2 expression was also investigated. CpG-ODN was found to up-regulate the expression of COX-2 in PC-3 cells in a dose- and time-dependent manner, but have little impact on COX-1 expression. Moreover, CpG-ODN also promoted nuclear translocation and activation of NF-kappa B, which appeared to be required for COX-2 induction by CpG-ODN. Overall, TLR9 up-regulates COX-2 expression in prostate cancer cells, at least partially through the activation of NF-kappa B, which may be implicated in tumor invasion and metastasis.

• 出版日期2010-4
• 单位中山大学