Differential expression of albumins and globulins of wheat flours of different technological qualities revealed by nanoUPLC-UDMSE

作者：Mayrinck Victorio Veronica Cristina; Souza Gustavo H M F; Barros Santos Millena Cristina; Vega Andres Rodriguez; Cameron L C^*; Larraz Ferreira Mariana Simoes

来源：Food Chemistry, 2018, 239: 1027-1036.

DOI：10.1016/j.foodchem.2017.07.049

• Domain prediction
• Gene ontology
• Ion mobility
• Proteomic
• Triticum aestivum

Soluble proteins were extracted from common wheat flour obtained from nine cultivars of different qualities and were analyzed by nanoUPLC and Ultra Definition Mass Spectrometry (UDMSE) label-free quantitative approach. Collectively, 5894 proteins were identified and quantified with 8 peptides/protein. A total of 414 proteins were found differentially expressed with 85% proteins not yet described in the literature, according to their biological function. Quality-related proteins, such as puroindolines and chaperones, notably involved in the gluten-protein folding process, were up-regulated in superior (SP) and medium (MD) flours qualities and down-regulated in the low (LW) ones. Domains related to the chaperones activity were also found up-regulated in SP and MD and down-regulated in LW. In this work, nanoUPLC-UDMSE analysis was effective to identify and quantify metabolic proteins, allowing a clear distinction of the wheat flours and providing new perspectives for evaluating the technological quality based on the low abundant proteins of wheat endosperm.

• 出版日期2018-1-12