Measurement of Glutathione in Normal Volunteers and Stroke Patients at 3T Using J-Difference Spectroscopy with Minimized Subtraction Errors

作者：An Li^*; Zhang Yan; Thomasson David M; Latour Lawrence L; Baker Eva H; Shen Jun; Warach Steven

来源：Journal of Magnetic Resonance Imaging, 2009, 30(2): 263-270.

DOI：10.1002/jmri.21832

• spectral registration
• J-difference
• subtraction errors
• stroke
• glutathione

Purpose: To develop and optimize a (1)H magnetic resonance spectroscopy (MRS) method for measuring brain glutathione (GSH) levels.
Materials and Methods: Phantom experiments and density operator simulations were performed to determine the optimal TE for measuring GSH at 3T using J-difference spectral editing. In vivo data collected from 11 normal volunteers (43 measurements) and five stroke patients (10 measurements) were processed using a new spectral alignment method (adaptive spectral registration).
Results: In phantom experiments and density operator simulations where relaxation effects were ignored, close to maximum GSH signal (2.95 ppm) was obtained at TE approximate to 131 msec with minimum N-acetyl-aspartate (NAA) signal interference. Using adaptive spectral, registration, GSH levels in healthy volunteers were found to be 1.20 /- 0.14 mM (mean /- standard deviation [SDI). GSH levels in stroke patients were found to be 1.19 /- 0.24 mM in lesion and 1.25 /- 0.19 mM in contralateral normal tissue. In comparison, the SDs were significantly larger when only the NAA singlet (2.01 ppm) was used as a navigator for spectral alignment.
Conclusion: Spectral editing using J-differences is a reliable method for measuring GSH levels in volunteers and stroke patients.

• 出版日期2009-8