Mitochondrial DNA control region sequences study in Saraiki population from Pakistan

作者：Hayat Sikandar^*; Akhtar Tanveer; Siddiqi Muhammad Hassan; Rakha Allah; Haider Naeem; Tayyab Muhammad; Abbas Ghazanfar; Ali Azam; Bokhari Syed Yassir Abbas; Tariq Muhammad Akram; Khan Fazle Majid

来源：Legal Medicine, 2015, 17(2): 140-144.

DOI：10.1016/j.legalmed.2014.10.010

• Mitochondrial DNA
• Hypervariable regions
• Haplotype
• Saraiki population
• Pakistan

The analysis of mitochondrial DNA (mtDNA) control region was carried in 85 unrelated Sariki individuals living in the different provinces of Pakistan. DNA was extracted from blood preserved in EDTA vacutainers. Hypervariable regions (HV1, HV2 & HV3) were PCR amplified and sequenced. Sequencing results were aligned and compared with revised Cambridge reference sequence (rCRS). The sequencing results showed presence of total 63 different haplotypes, 58 of them are unique and 05 are common haplotypes shared by more than one individual. The most common haplotype observed was (W6) with a frequency 12.9% of population sample. The Saraiki population was detected with genetic diversity (0.9570) and power of discrimination (0.9458). This study will be beneficial for forensic casework.

• 出版日期2015-3