A novel enzymatic method for synthesis of glycopeptides carrying natural eukaryotic N-glycans

作者：Xu, Yangyang; Wu, Zhigang; Zhang, Peiru; Zhu, He; Zhu, Hailiang; Song, Qitao; Wang, Li; Wang, Faxing; Wang, Peng George^*; Cheng, Jiansong^*

来源：Chemical Communications, 2017, 53(65): 9075-9077.

DOI：10.1039/c7cc04362g

• CHEMOENZYMATIC SYNTHESIS
• GLYCOPROTEIN-SYNTHESIS
• LINKED OLIGOSACCHARIDES
• PROTEIN GLYCOSYLATION
• SUGARS

A novel enzymatic approach was developed for facile production of glycopeptides carrying natural eukaryotic N-glycans. In this approach, peptides can be GlcNAcylated at one or two natural N-glycosylation sites via two-step enzymatic reactions catalyzed by an evolved N-glycosyltransferase (ApNGTQ469A) and a glucosamine N-acetyltransferase (GlmA), respectively. The resulting GlcNAc-peptides were further modified by an endo-beta-N-acetylglucosaminidase M mutant (EndoM(N175Q)) to generate glycopeptides. In three steps of enzymatic catalysis, glycopeptides carrying complex-type N-glycans can be efficiently synthesized.

• 出版日期2017-8-21
• 单位药物化学生物学国家重点实验室; 南开大学