Neuronal IL-17 receptor upregulates TRPV4 but not TRPV1 receptors in DRG neurons and mediates mechanical but not thermal hyperalgesia

作者：von Banchet Gisela Segond^*; Boettger Michael K; Koenig Christian; Iwakura Yoichiro; Braeuer Rolf; Schaible Hans Georg

来源：Molecular and Cellular Neuroscience, 2013, 52: 152-160.

DOI：10.1016/j.mcn.2012.11.006

• TRPV1
• TRPV4
• IL-17A
• IL-17RA
• ERK
• NF kappa B

In addition to the proinflammatory cytokines tumor necrosis factor-alpha, interleukin-6 and interleukin-1 beta, the cytokine interleukin-17 (IL-17) is considered an important mediator of autoimmune diseases such as rheumatoid arthritis. Because tumor necrosis factor-alpha and interleukin-1 beta have the potential to influence the expression of transduction molecules such as transient receptor potential vanilloid 1 (TRPV1) in dorsal root ganglion (DRG) neurons and thus to contribute to pain we explored in the present study whether IL-17A activates DRG neurons and influences the expression of TRPV1. The IL-17A receptor was visualized in most neurons in dorsal root ganglion (DRG) sections as well as in cultured DRG neurons. Upon long-term exposure to IL-17A, isolated and cultured rat DRG neurons showed a significant upregulation of extracellular-regulated kinase (ERK) and nuclear factor kappa B (NF kappa B). Long-term exposure of neurons to IL-17A did not upregulate the expression of TRPV1. However, we found a pronounced upregulation of transient receptor potential vanilloid 4 (TRPV4) which is considered a candidate transduction molecule for mechanical hyperalgesia. Upon the injection of zymosan into the paw, IL-17A-deficient mice showed less mechanical hyperalgesia than wild type mice but thermal hyperalgesia was not attenuated in IL-17A-deficient mice. These data show, therefore, a particular role of IL-17 in mechanical hyperalgesia, and they suggest that this effect is linked to an activation and upregulation of TRPV4.

• 出版日期2013-1
• 单位河北医科大学