A Phase I New Approaches to Neuroblastoma Therapy Study of Buthionine Sulfoximine and Melphalan With Autologous Stem Cells for Recurrent/Refractory High-Risk Neuroblastoma

作者：Villablanca Judith G^*; Volchenboum Samuel L; Cho Hwangeui; Kang Min H; Cohn Susan L; Anderson Clarke P; Marachelian Araz; Groshen Susan; Tsao Wei Denice; Matthay Katherine K; Maris John M; Hasenauer Charlotte E; Czarnecki Scarlett; Lai Hollie; Goodarzian Fariba; Shimada Hiro; Reynolds Charles Patrick

来源：Pediatric Blood and Cancer, 2016, 63(8): 1349-1356.

DOI：10.1002/pbc.25994

• buthionine sulfoximine
• melphalan
• neuroblastoma
• Phase 1
• transplant

Background. Myeloablative therapy for high-risk neuroblastoma commonly includes melphalan. Increased cellular glutathione (GSH) can mediate melphalan resistance. Buthionine sulfoximine (BSO), a GSH synthesis inhibitor, enhances melphalan activity against neuroblastoma cell lines, providing the rationale for a Phase 1 trial of BSO-melphalan. Procedures. Patients with recurrent/resistant high-risk neuroblastoma received BSO (3 gram/m(2) bolus, then 24 grams/m(2)/day infusion days -4 to -2), with escalating doses of intravenous melphalan (20-125 mg/m(2)) days -3 and -2, and autologous stem cells day 0 using 3 + 3 dose escalation. Results. Among 28 patients evaluable for dose escalation, one dose-limiting toxicity occurred at 20 mg/m(2) melphalan (grade 3 aspartate aminotransferase/alanine aminotransferase) and one at 80 mg/m(2) (streptococcal bacteremia, grade 4 hypotension/pulmonary/hypocalcemia) without sequelae. Among 25 patients evaluable for response, there was one partial response (PR) and two mixed responses (MRs) among eight patients with prior melphalan exposure; one PR and three MRs among 16 patients without prior melphalan; one stable disease with unknown melphalan history. Melphalan pharmacokinetics with BSO were similar to reports for melphalan alone. Melphalan C-max for most patients was below the 10 mu M concentration that showed neuroblastoma preclinical activity with BSO. Conclusions. BSO (75 gram/m(2)) with melphalan (125 mg/m(2)) is tolerable with stem cell support and active in recurrent/refractory neuroblastoma. Further dose escalation is feasible and may increase responses.

• 出版日期2016-8