Development and Validation of an Analytical Method by UV Spectrophotometry for the Quantitative Determination of the Anti-neoplastic beta-lapachone

作者：Presmich Geisiane M A; Rolim Larissa A; Cavalcanti Otavio R B R; Silva Keyla E R; Rolim Neto Pedro J^*

来源：Latin American Journal of Pharmacy, 2010, 29(5): 645-650.

• beta-lapachone
• oncology
• quantification
• Spectroscopy
• Validation

"Development and Validation of an Analytical Method by UV Spectrophotometry for the Quantitative Determination of the Anti-neoplastic beta-lapachone". beta-lapachone is a natural ortonaftoquinone extract of the Bignoniaceae plants, as Ipe Roxo (Tabebuia avellanedae Lor), tree native to Brazil, or can be synthesized from its isomer lapachol. beta-lapachone has demonstrated a range of pharmacological activities including antibacterial, antifungal, trypanocidal, antiviral and anticancer. There are no reports in the literature of a spectrophotometric method for quantification of beta-lapachone. The present research describes the development of a simple, economical, fast and accurate for the quantification of this drug. The method developed is the solubilization of beta-lapachone in absolute ethanol with subsequent dilution in the solvent system of absolute ethyl alcohol: purified water for quantification by UV detector. The method was validated according International Conference on harmonization (ICH Q2A /ICH Q2B) and National Health Surveillance Agency of Brazil (RE 899/03) parameters. The results show that the validation method is robust, linear, sensitive, precise and accurate within the parameters assessed.

• 出版日期2010-8