A 94-bp Deletion of Anthocyanidin Synthase Gene in Acyanic Flower Lines of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.]

作者：Shimizu Keiichi; Ohnishi Nanako; Morikawa Noriyuki; Ishigami Ai; Otake Saeko; Rabah Isselmou Ould; Sakata Yusuke; Hashimoto Fumio^*

来源：Journal of the Japanese Society for Horticultural Science, 2011, 80(4): 434-442.

DOI：10.2503/jjshs1.80.434

• anthocyanidin synthase
• anthocyanin
• Eustoma grandiflorum
• flower color
• mutation

We studied the genomic sequence of the anthocyanidin synthase (ANS) gene in acyanic flower lines of lisianthus and detected a 94-bp deletion of the sequence in comparison with that of cyanic flower lines. Cross-pollination was made between acyanic and cyanic flower lines to yield F-1 progeny, followed by its self-pollination. The segregated individuals between cyanic and acyanic flowers among the F-2 population were of 24 and 8, respectively (3 : 1), which suggested that one gene of the F-1 hybrid was unequivocally involved in anthocyanin production. The homozygous ANS genes that have a set of 94-bp deletion co-segregated a recessive phenotype of white color in the F-2 population. Genomic PCR of the ANS genes of the 14 independent lisianthus lines of acyanic flowers, such as white, yellow, pale yellow, and pale green flowers, revealed that all possessed a homozygous 94-bp deletion. The data support that the acyanic flower colors are associated with a 94-bp deletion in the ANS gene as a result of the loss of anthocyanin accumulation.

• 出版日期2011-10