NOTCH1 mutation and its prognostic significance in Chinese chronic lymphocytic leukemia: a retrospective study of 317 cases

作者：Zou, Yixin; Fan, Lei; Xia, Yi; Miao, Yi; Wu, Wei; Cao, Lei; Wu, Jiazhu; Zhu, Huayuan; Qiao, Chun; Wang, Li; Xu, Wei^*; Li, Jianyong^*

来源：Cancer Medicine, 2018, 7(5): 1689-1696.

DOI：10.1002/cam4.1396

• Chronic lymphocytic leukemia
• cytogenetics
• molecular biology
• NOTCH1 mutations
• prognosis

The proto-oncogene NOTCH1 is frequently mutated in around 10% of patients with chronic lymphocytic leukemia (CLL). This study analyzed NOTCH1 mutation status of 317 Chinese patients with CLL by Sanger sequencing. The frequencies of NOTCH1 mutation in the PEST (proline (P), glutamic acid (E), serine (S), threonine (T)-rich protein sequence) domain and the 3' untranslated regions (UTR) were 8.2% and 0.9%, with the most frequent mutation being c.7541_7542delCT and c.*371A>G, respectively. Clinical and biological associations were determined including NOTCH1 mutations with advanced stage (Binet stage, P= 0.010), unmutated immunoglobulin heavy-chain variable region (IGHV) gene (P < 0.001) and trisomy 12 (+12) (P = 0.014). NOTCH/-mutated patients had lower CD20 expression intensity than NOTCHI-unmutated patients (P = 0.029). In addition, NOTCH1-mutated patients had shorter overall survival (OS) (P = 0.002) and treatment-free survival (TFS) (P = 0.002) than NOTCH1-unmutated patients, especially for patients with NOTCHI c.7541_7542deICT and/or c.*371A>G mutations. Patients with both mutated NOTCHI and unmutated IGIW had shorter OS (P < 0.001) and TFS (P < 0.001) than those with unmutated NOTCHI or mutated IGHV. These data provide a comprehensive view of the clinical relevance and prognostic impact of NOTCHI mutations on Chinese patients with CLL.

• 出版日期2018-5
• 单位南京医科大学