A Novel Nonhuman Primate Model of Cigarette Smoke-Induced Airway Disease

作者：Polverino Francesca; Doyle Eisele Melanie; McDonald Jacob; Wilder Julie A; Royer Christopher; Laucho Contreras Maria; Kelly Emer M; Divo Miguel; Pinto Plata Victor; Mauderly Joe; Celli Bartolome R; Tesfaigzi Yohannes; Owen Caroline A^*

来源：American Journal Of Pathology, 2015, 185(3): 741-755.

DOI：10.1016/j.ajpath.2014.11.006

Small animal models of chronic obstructive pulmonary disease (COPD) have several limitations for identifying new therapeutic targets and biomarkers for human COPD. These include a pulmonary anatomy that differs from humans, the limited airway pathologies and lymphoid aggregates that develop in smoke-exposed mice, and the challenges associated with serial biological sampling. Thus, we assessed the utility of cigarette smoke (CS) exposed cynomolgus macaque as a nonhuman primate (NHP) large animal model of COPD. Twenty-eight NHPs were exposed to air or CS 5 days per week for up to 12 weeks. Bronchoalveolar lavage and pulmonary function tests were performed at intervals. After 12 weeks, we measured airway pathologies, pulmonary inflammation, and airspace enlargement. CS-exposed NHPs developed robust mucus metaplasia, submucosal gland hypertrophy and hyperplasia, airway inflammation, peribronchial fibrosis, and increases in bronchial Lymphoid aggregates. Although CS-exposed NHPs did not develop emphysema over the study time, they exhibited pathologies that precede emphysema development, including increases in the following: i) matrix metalloproteinase-9 and proinflammatory mediator levels in bronchoalveolar lavage fluid, ii) lung parenchymal Leukocyte counts and Lymphoid aggregates, iii) lung oxidative stress levels, and iv) alveolar septal cell apoptosis. CS-exposed NHPs can be used as a model of airway disease occurring in COPD patients. Unlike rodents, NHPs can safely undergo longitudinal sampling, which could be useful for assessing novel biomarkers or therapeutics for COPD.

• 出版日期2015-3
• 单位河北医科大学