Astrocytes facilitate the growth and differentiation of co-cultured mesenchymal stem cells

作者：Chu, Qian^*; Yu, Zhiyuan; Zhang, Suming; Yu, Shiying

来源：Journal of Huazhong University of Science and Technology-Medical Sciences, 2008, 28(3): 333-336.

DOI：10.1007/s11596-008-0323-2

• mesenchymal stem cells
• astrocyte
• differentiation
• neural like cells

The effect of astrocytes on the growth and differentiation of co-cultured mesenchymal stem cells (MSCs) was studied. MSCs of Wistar rats were isolated, cultured and labeled with Hoechst33342. The labeled MSCs were co-cultured with astrocytes in DMEM/F12/10%FBS. The growth status and morphologic change of MSCs were observed. The expression of specific protein NeuN, GFAP and CNP was detected by using immunofluorescence staining. The results showed that the two kinds of co-cultured cells grew well. Some labeled MSCs stretched out long processes with spin, triangle and star shapes. Immunofluorescence stain showed that these cells expressed NeuN, GFAP or CNP. It was suggested that astrocytes could promote the prolifevation of co-cultured MSCs and induce them to differentiate into neural like cells.

• 出版日期2008-6
• 单位华中科技大学