Axial ligation of the high-potential heme center in an Arabidopsis cytochrome b561

作者：Desmet Filip; Berczi Alajos; Zimanyi Laszlo; Asard Han; Van Doorslaer Sabine^*

来源：FEBS LETTERS, 2011, 585(3): 545-548.

DOI：10.1016/j.febslet.2011.01.006

• Double mutant
• EPR
• Cytochrome b561
• Optical absorption spectroscopy
• Resonance Raman spectroscopy

Arabidopsis has four putative, di-heme cytochrome b561 proteins, including one localized in the tonoplast (TCytb). From a comparative electron paramagnetic resonance (EPR), UV-Vis absorption and resonance Raman study, on wild type, H83A/H156A-TCytb and H83L/H156L-TCytb double mutants, it follows that the H83 and H156 residues are binding one of the two hemes. These measurements show that the high-potential heme site is situated at the cytoplasmic side of the membrane and allow the unambiguous differentiation between two models on the heme localization in cytochrome b561 proteins.

• 出版日期2011-2-4