Development and validation of a LC-MS/MS method based on a new 96-well Hybrid-SPE (TM)-precipitation technique for quantification of CYP450 substrates/metabolites in rat plasma

作者：Ardjomand Woelkart Karin; Kollroser Manfred; Li Li; Derendorf Hartmut; Butterweck Veronika; Bauer Rudolf^*

来源：Analytical and Bioanalytical Chemistry, 2011, 400(8): 2371-2381.

DOI：10.1007/s00216-010-4618-3

• Hybrid-SPE (TM) precipitation
• Validation
• Phospholipids
• CYP450

A rapid and selective high-throughput HESI-LC-MS/MS method for determining eight cytochrome P450 probe drugs in one-step extraction and single run was developed and validated. The four specific probe substrates midazolam, dextromethorphan, tolbutamide, theophylline and their metabolites 1-hydroxymidazolam, dextrorphan, hydroxyl(methyl)tolbutamide, 1,3-dimethyluric acid, together with the deuterated internal standards, were extracted from rat plasma using a novel 96-well Hybrid-SPE (TM)-precipitation technique. The bioanalytical assay was based on reversed phase liquid chromatography coupled with tandem mass spectrometry in the positive ion mode using selected reaction monitoring for drug (-metabolite) quantification. All analytes were separated simultaneously in a single run that lasted less than 11 min. The intra- and inter-day precisions for all eight substrates/metabolites were 1.62-12.81% and 2.09-13.02%, respectively, and the relative errors (accuracy) for the eight compounds ranged from -9.62% to 7.48% and -13.84% to 8.82%. Hence, the present method provides a robust, fast and reproducible analytical tool for the evaluation of four major drug metabolising cytochrome P450 (3A4, 2C9, 1A2 and 2D6) activities with a cocktail approach in rats to clarify herb-drug interactions. The method can be used as a basic common validated high-throughput analytical assay for in vivo interaction studies.

• 出版日期2011-6