Healthy offspring from freeze-dried mouse spermatozoa held on the International Space Station for 9 months

作者：Wakayama Sayaka^*; Kamada Yuko; Yamanaka Kaori; Kohda Takashi; Suzuki Hiromi; Shimazu Toru; Tada Motoki N; Osada Ikuko; Nagamatsu Aiko; Kamimura Satoshi; Nagatomo Hiroaki; Mizutani Eiji; Ishino Fumitoshi; Yano Sachiko; Wakayama Teruhiko^*

来源：Proceedings of the National Academy of Sciences of the United States of America, 2017, 114(23): 5988-5993.

DOI：10.1073/pnas.1701425114

• International Space Station
• preservation
• freeze-dry
• spermatozoa
• fertilization

If humans ever start to live permanently in space, assisted reproductive technology using preserved spermatozoa will be important for producing offspring; however, radiation on the International Space Station (ISS) is more than 100 times stronger than that on Earth, and irradiation causes DNA damage in cells and gametes. Here we examined the effect of space radiation on freeze-dried mouse spermatozoa held on the ISS for 9 mo at -95 degrees C, with launch and recovery at room temperature. DNA damage to the spermatozoa and male pronuclei was slightly increased, but the fertilization and birth rates were similar to those of controls. Next-generation sequencing showed only minor genomic differences between offspring derived from space-preserved spermatozoa and controls, and all offspring grew to adulthood and had normal fertility. Thus, we demonstrate that although space radiation can damage sperm DNA, it does not affect the production of viable offspring after at least 9 mo of storage on the ISS.

• 出版日期2017-6-6