Assay of superoxide dismutase activity in a plate assay using WST-1

作者:Peskin Alexander V; Winterbourn Christine C*
来源:Free Radical Biology and Medicine, 2017, 103: 188-191.
DOI:10.1016/j.freeradbiomed.2016.12.033

摘要

One of the most convenient methods for measuring superoxide dismutase activity is a plate assay using xanthine oxidase and the water soluble tetrazolium WST-1. For reliable results with WST-1, certain aspects of the procedure need to be adhered to. This article describes an appropriate protocol that minimizes sources of variability.

  • 出版日期2017-2