Analysis of enniatins and beauvericin by LC-MS/MS in wheat-based products

作者：Stanciu Oana; Juan Cristina; Miere Doina; Loghin Felicia; Manes Jordi

来源：CyTA-Journal of Food, 2017, 15(3): 433-440.

DOI：10.1080/19476337.2017.1288661

• Emerging mycotoxins
• LC-MS
• MS
• method validation
• cereal-based products
• Micotoxinas emergentes
• LC-MS
• MS
• validacion
• productos elaborados con trigo

Due to the matrix complexity for wheat-based products, a comparative study of different rapid extraction procedures was performed for the extraction of enniatins (ENA, ENA1, ENB, ENB1) and beauvericin in flour, pasta, breakfast cereals, and biscuits. Three different approaches were studied during the extraction and purification steps (shaker, Ultra-Turrax, and QuEChERS) for each matrix. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) with electrospray source working in a positive mode was used. For the analysis of the five mycotoxins, the three methods were tested in terms of recovery, matrix effect, and sensibility, concluding that Ultra-Turrax extraction was the most competent method. The applicability of the validated method was demonstrated by analyzing 16 commercial samples from Romania. RESUMENDebido a la complejidad de los productos elaborados con trigo, se ha realizado un estudio comparativo de diferentes procesos de extraccion rapida de eniatinas (ENA, ENA1, ENB, ENB1) y beauvericina en harina, pasta, cereales para el desayuno y galletas. Se estudiaron tres procedimientos (agitacion, Ultra-Turrax, QuEChERS) en cada matriz con distintos pasos de extraccion y purificacion. Se utilizo la cromatografia liquida acoplada a espectrometria de masas en tandem (LC-MS/MS) con fuente de electroespray en modo positivo. Los tres metodos fueron estudiados para el analisis de las cinco micotoxinas, en terminos de recuperacion, efecto matriz y sensibilidad, concluyendo que el metodo de extraccion con Ultra-Turrax fue el que mejores resultados proporciono. La aplicabilidad del metodo validado se demostro con el analisis de 16 muestras comercializadas en Rumania.

• 出版日期2017