Antimicrobial activity, effects on Streptococcus mutans biofilm and interfacial bonding of adhesive systems with and without antibacterial agent

作者：Andre Carolina Bosso^*; Figueiredo Almeida Gomes Brenda Paula; Duque Thais Mageste; Rosalen Pedro Luis; Chan Daniel Chi Ngai; Bovi Ambrosano Glaucia Maria; Giannini Marcelo

来源：International Journal of Adhesion and Adhesives, 2017, 72: 123-129.

DOI：10.1016/j.ijadhadh.2016.10.011

• Antibacterial adhesives
• Dentine
• Hybrid layer

Objectives: This study evaluated the antimicrobial activity (by agar disk diffusion test, AD), viability of S. mutans biofilm (VB), and effect on resin-dentin interface (RDI) of six adhesive systems.
Methods: Three adhesives containing antibacterial components (Gluma 2Bond (G2B)/glutaraldehyde, Clearfil SE Protect (CSP)/MDPB and Peak Universal Bond (PUB)/chlorhexidine) and the corresponding adhesives with similar composition, but without antibacterial agents (Gluma Comfort Bond, GCB; Clearfil SE Bond, CSB and Peak LC Bond, PLB) were evaluated. AD was determined measuring the extent of halo formation following application of adhesives and control groups (light cured or not) to cultures of four strict anaerobic and four facultative bacteria. For VB, a UA159 biofilm was grown on adhesive-coated hydroxyapatite discs for five days, collected and processed to count the number of viable cells. For RDI analysis, adhesives were applied according to manufacturers' recommendations and teeth were restored with resin composite, sectioned to obtain bonded slices and visualized by SEM.
Results: An inhibition halo was observed for G2B (strict anaerobic/light cured and not light cured), CSP (strict anaerobic and facultative/light cured and not light cured) and PUB (strict anaerobic and facultative/not light cured). PUB when light cured produced an inhibition halo on L. casei and S. mutans only. G2B and CSP significantly reduced the viability of S. mutans. Adhesives containing antimicrobial compounds had no detectable effect on RDI.
Conclusion: The MDPB-containing bonding agent showed better results of inhibition for all oral pathogens tested and a decrease of viability of Streptococcus mutans biofilm, among the adhesives tested.

• 出版日期2017-1