An integrated map of p53-binding sites and histone modification in the human ENCODE regions

作者：Kaneshiro Kiyofumi; Tsutsumi Shuichi; Tsuji Shingo; Shirahige Katsuhiko; Aburatani Hiroyuki^*

来源：Genomics, 2007, 89(2): 178-188.

DOI：10.1016/j.ygeno.2006.09.001

• TP53 protein
• historic
• chromatin
• oligonucleotide microarrays
• HCT116 cells
• human

TP53 (tumor protein p53; p53) regulates its target genes under various cellular stresses. By combining chromatin ii-nmunoprecipitation with oligonucleotide microarrays, we have mapped binding sites of p53 (p53-BS) in the genome of HCT 116 human colon carcinoma cells, along with those of acetylated H3, acetylated H4, and methylated H3-K4. We analyzed a 30-Mb portion of the human genome selected as a representative model by the ENCODE Consortium. In the region, we found 37 p53-13S, of which the p53-binding motif was present in 32 (86%). Acetylated historic H3 and H4 were detected at 14 (38%) and 33 (89%) of the p53-BS, respectively. A significant portion (58%) of H4 acetylation in the p53-13S was not accompanied by H3 acetylation. Acetyl H3 were preferentially located at the 5' and 3' ends of genes, whereas acetyl H4 were distributed widely across the genome. These results provide novel insights into how p53 binding coordinates with historic modification in human.

• 出版日期2007-2