Down-regulation of miR-92a inhibits cell proliferation and invasion but induces apoptosis of gastric cancer by regulating FBXW7

作者：Zhou, Haiyang^*; Zhang, Yong; Wei, Xueming; Zheng, Aimin; Dai, Dajiang; Zhang, Haihong; Cao, Houjun

来源：International Journal of Clinical and Experimental Pathology, 2016, 9(4): 4283-4291.

• Gastric cancer
• miR-92a
• cell proliferation
• cell apoptosis
• cell invasion

This study aimed to investigate the potential roles of miR-92a expression in the development and invasion of gastric cancer, and to illustrate the potential mechanism. Expression of miR-92a in gastric cancer SGC7901 cells were analyzed using RT-PCR. MTT assay, Transwell assay, and Annexin V-FITC were used to analyze the influences of miR-92a expression on tumor cell viability, invasion, and apoptosis respectively. Western blot was used to detect the cell apoptosis-related protein expression in SGC7901 cells. Compared to the normal gastric mucosa epithelial 3T3 cells, miR-92a was highly expressed in SGC7901 cells. Accordingly, miR-92a suppression significantly decreased the cell viability, induced apoptosis, and suppressed cell invasion. Moreover, the suppressed miR-92a significantly increased the expression of FBXW7, but decreased the cycline E and c-myc expression in SGC7901 cells. Taken together, our study suggested that miR-92a suppression may play certain inhibit roles in the development and invasion of gastric cancer through involving in biological processes including proliferation, apoptosis, and invasion by negatively regulating FBXW7 and its downstream protein of cycline E and c-myc.

• 出版日期2016
• 单位中国人民解放军空军总医院