科研之友
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摘要
Annual gonad hormonal profile of wild matured mahseer (29 males and 23 female) averaging in weight of 0.95 +/- 0.26 and 1.19 +/- 0.23 kg for males and females, respectively were investigated from November 2007 to November 2008 using enzyme-linked immunosorbent assay (ELISA) technique. Blood was collected from caudal vein monthly and plasma separation by centrifugation was done to measure reproductive hormones: 17 beta-estradiol (E-2), testosterone (T) and 11-keto-testosterone (11KT). Gonads were sampled for histology processing to observe their maturity. Highest T level in females and males were recorded at 0.22 +/- 0.016 and 0.88 +/- 0.014 ng/ml, respectively. The 11KT showed several peaks and highest value was noted at 0.7 +/- 0.018 ng/ml in November 2008. The female E-2 initially was at 1.48 +/- 0.16 ng/ml and significantly increased (P < 0.05) to 1.53 +/- 0.39 ng/ml in November 2008. Ovaries were laden with several oocyte stages in the entire samples while testes gonad showed high level of spermatids throughout the year. Changes in plasma level of the gonadal hormones were correlated with the ovarian and testes maturities. In conclusion, the study suggest that mahseer can be categorized as asynchronize and multiple spawner. The information gathered is important for appropriate breeding and conservation programs of the Malaysian mahseer.
- 出版日期2011-1-1
