Autophagy-related gene16L2, a potential serum biomarker of multiple sclerosis evaluated by bead-based proteomic technology

作者：Yin, Linlin^*; Liu, Jianghong; Dong, Huiqing; Xu, Erhe; Qiao, Yuchen; Wang, Lin; Zhang, Lan; Jia, Jianping; Li, Lin; Geng, Xingchao

来源：Neuroscience Letters, 2014, 562: 34-38.

DOI：10.1016/j.neulet.2013.12.070

• Atg16L2
• multiple sclerosis
• proteomic technology
• T cells

Multiple sclerosis (MS) is an autoimmune disease characterized by neuroinflammation and demyelination that are mediated by T cells. The prolonged survival of autoreactive T cells acts as a primary event to trigger an inflammatory cascade that mediates myelin loss and clinical relapse in MS. Recently, T cell survival has been shown to be modulated by the autophagy-related gene (Atg). In the present study, we performed bead fractionation/matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analyses using serum from 54 MS patients and 55 healthy controls. Eleven peptides were significantly different between the two groups with one being identified as a fragment of Atg16L2. Then the decreased levels of Atg16L2 peptides in MS patients were validated by immunoblotting and real-time PCR. As the Atg12-Atg5.Atg16 multimeric complex plays an essential role in autophagy, our results suggest that Atg16L2 may play an important role in autophagy of T cells and serve as a potential biomarker to predict clinical relapse of MS.

• 出版日期2014-3-6
• 单位北京市老年病医疗研究中心; 首都医科大学