Differential accumulation of the transcripts of 22 novel protein kinase genes in Arabidopsis thaliana

作者：Thummler F^*; Kirchner M; Teuber R; Dittrich P

来源：Plant Molecular Biology, 1995, 29(3): 551-565.

DOI：10.1007/BF00020984

• plant protein kinases
• polymerase chain reaction (PCR)
• light regulation
• signal transduction
• tissue-specific expression
• Southern blot

22 novel members of the Arabidopsis thaliana protein kinase family (AKs) were identified by using degenerate oligonucleotide primers directed to highly conserved amino acid sequences of the protein kinase (PK) catalytic domain. Of these 22 genes, 16 turned out to carry intron sequences. Homologies of AK sequences were detected to S-locus receptor protein kinases (SRKs) from Brassica spp., to SRK-like PKs from maize and A. thaliana and to several other receptor PKs from A. thaliana. Sequence similarity was also detected to Ca2+-dependent PKs (CDPKs) from rape and soybean, to SNF1 and to CDC2 homologues. The genomic organization and the accumulation of the mRNAs from these 22 AK genes were investigated.

• 出版日期1995-11