Microarray and suppression subtractive hybridization analyses of gene expression in hybrid poplar (Populus alba x Populus tremula var. glandulosa) cell suspension cultures after exposure to NaCl

作者：Bae Eun Kyung; Lee Hyoshin^*; Lee Jae Soon; Noh Eun Woon; Choi Young Im; Lee Byung Hyun; Choi Dong Woog

来源：Plant Physiology and Biochemistry, 2012, 58: 151-158.

DOI：10.1016/j.plaphy.2012.04.015

• Gene expression
• Microarray
• Populus
• Salt stress
• SSH

The gene expression profiles of hybrid poplar (Populus alba x Populus tremula var. glandulosa) cells in suspension culture after exposure to salinity (NaCl) induced stress were examined by constructing two suppression subtractive hybridization (SSH) libraries. cDNA from non-treated cells was used as a driver and cDNA samples from cell suspension cultures exposed to 150 mM NaCl for 2 or 10 h were used as testers. Randomly selected clones from each SSH library were sequenced and 727 high-quality expressed sequence tags (ESTs) were obtained and analyzed. Four novel ESTs were identified. Between the two libraries, 542 unique SSH clones were selected for placement on a cDNA microarray. In total, 18 differentially expressed genes were identified with 4 and 12 genes being significantly differentially expressed 2 and 10 h after the treatment, respectively. Genes related to metabolism and protein synthesis and several genes whose protein products are implicated in salt or other abiotic stress-related responses were expressed in the salt-stressed cells.

• 出版日期2012-9