Ultrasensitive UPLC-MS-MS Method for the Quantitation of Etheno-DNA Adducts in Human Urine

作者：Cui, Shiwei; Li, Haibin; Wang, Shaojia; Jiang, Xiao; Zhang, Shusheng; Zhang, Rongjie; Sun, Xin^*

来源：International Journal of Environmental Research and Public Health, 2014, 11(10): 10902-10914.

DOI：10.3390/ijerph111010902

• UPLC-MS/MS
• 1,N-6-ethenodeoxyadenosine
• 3,N-4-ethenodeoxycytidine
• lipid peroxidation
• human biomonitoring

Etheno-DNA adducts are generated from the metabolism of exogenous carcinogens and endogenous lipid peroxidation. We and others have previously reported that 1,N-6-ethenodeoxyadenosine (epsilon dA) and 3,N-4-ethenodeoxycytidine (epsilon dC) are present in human urine and can be utilized as biomarkers of oxidative stress. In this study, we report a new ultrasensitive UPLC-ESI-MS/MS method for the analysis of epsilon dA and epsilon dC in human urine, capable of detecting 0.5 fmol epsilon dA and 0.3 fmol epsilon dC in 1.0 mL of human urine, respectively. For validation of the method, 20 human urine samples were analyzed, and the results revealed that the mean levels of epsilon dA and epsilon dC (SD) fmol/mu mol creatinine are 5.82 +/- 2.11 (range 3.0-9.5) for epsilon dA and 791.4 +/- 328.8 (range 116.7-1264.9) for epsilon dC in occupational benzene-exposed workers and 2.10 +/- 1.32 (range 0.6-4.7) for epsilon dA and 161.8 +/- 200.9 (range 1.8-557.5) for epsilon dC in non-benzene-exposed workers, respectively. The ultrasensitive detection method is thus suitable for applications in human biomonitoring and molecular epidemiology studies.

• 出版日期2014-10
• 单位中国疾病预防控制中心职业卫生与中毒控制所; 中国疾病预防控制中心; 郑州大学; 华北理工大学; 河南省疾病预防控制中心