The effects of alpha-defensin 1 on electrical field stimulation-induced contraction of rat bladders

作者：Lee Shin Young; Do Kim Kyung; Park Soo Hyun; Lee Moo Yeol; Kim Hae Jong; Han June Hyun; Myung Soon Chul^*

来源：European Journal of Pharmacology, 2013, 715(1-3): 420-423.

DOI：10.1016/j.ejphar.2013.03.032

• alpha-defensin 1 (HNP1)
• Rat bladder muscle
• EFS (Electrical field stimulation)
• Bladder overactivity
• Purinergic contraction

This study was designed to investigate the effects of alpha-defensin 1 on electrical field stimulation (EFS)-induced contractions in isolated rat bladder detrusor muscles. We evaluated the effects of alpha-defensin 1 (50 pM similar to 5 nM) on EFS-induced contractions in the detrusor smooth muscles from 35 rats (2-30 Hz). Bladder strips were pretreated with alpha-defensin 1 and then changes of contractions by adenosine 5%26apos;-triphosphate (ATP) were observed. Moreover, after pretreatment with alpha-defensin 1 for 10 min, changes in concentration-response curves to hydrogen peroxide (H2O2) were investigated. Alpha-defensin 1 has increased EFS-induced contractions, significantly, and the contractile response to ATP (1,2,5,10 mM) was also increased significantly when strips were pretreated with alpha-defensin 1. In addition, alpha-defensin 1 increased H2O2-induced contractions. The present study demonstrates that alpha-defensin 1 increases EFS-induced contractions of rat detrusor muscles via purinergic contraction coupled with the Rho kinase pathway.

• 出版日期2013-9-5