Identification and fermentation optimization of protopectinase-overproducing strain Aspergillus niger CD-01 for pectin production

作者：Xia Jin lan^*; Meng Hao; Wang Run min; Zhang Cheng gui; Xiong Jing; Nie Zhen yuan; Qiu Guan zhou

来源：Journal of Central South University of Technology, 2009, 16(1): 53-60.

DOI：10.1007/s11771-009-0009-3

• Aspergilllus niger
• pectin production
• protopectinase
• citrus peel
• fermentation optimization

In order to solve the citrus peel resource waste problem and minimize the drawbacks of chemical extraction of pectin, a protopectinase-overproducing strain CD-01 for pectin production was isolated from a pit soil dumped with perished orange in Changde City, Hunan Province of China. The strain CD-01 had the same morphology and 28S rRNA gene sequence (FJ184995) as that of Aspergillus niger (ATCC 64028). It was thus identified and named as Aspergillus niger CD-01. The fermentation condition was optimized based on L-9(3(4)) orthogonal experimental design and the variances analyses. The results show that the optimal condition for producing pectin is as follows: time 36 h, temperature 35 degrees C, pH 5, and urea as the nitrogen source. Under this condition, the pectin yield can reach up to 24.5%. This shows a great potential of Aspergillus niger CD-01 in pectin extraction from citrus.

• 出版日期2009-2
• 单位中南大学