A novel electrochemiluminescent immunosensor based on the quenching effect of aminated graphene on nitrogen-doped carbon quantum dots

作者：Zhou, Jing; Han, Tongqian; Ma, Hongmin; Yan, Tao; Pang, Xuehui; Li, Yueyun; Wei, Qin^*

来源：Analytica Chimica Acta, 2015, 889: 82-89.

DOI：10.1016/j.aca.2015.07.018

• Resonance energy transfer
• Nitrogen-doped carbon quantum dots
• Alpha fetoprotein
• Aminated graphene

Nitrogen-doped carbon quantum dots (N-CQDs) with an average diameter of 2 nm were synthesized by carbonization of diethylene triamine pentacetate acid (DTPA). The simple prepared N-CQDs showed excellent electrochemiluminescence (ECL) property and were used as luminophors to fabricate a sandwich-type ECL immunosensor. Aminated graphene (NH2-G) was also synthesized and used as a label of secondary antibody. The labeled NH2-G could effectively quench the ECL of N-CQDs modified on electrodes due to ECL resonance energy transfer (ERET). Immunological recognition which induced ECL quenching enabled the quantitative determination of biomarkers. Alpha fetoprotein (AFP) was selected as a model analyte to investigate the analytical performance of the proposed immunosensor. Under optimal conditions, a good linear relationship between ECL intensity and the logarithm of AFP concentration was obtained in the range of 0.01-100 ng mL(-1) with the detection limit of 3.3 pg mL(-1). The proposed ECL immunosensor showed good stability, acceptable selectivity and reproducibility.

• 出版日期2015-8-19
• 单位济南大学; 山东大学; 山东理工大学