Determinants of Versican-V1 Proteoglycan Processing by the Metalloproteinase ADAMTS5

作者：Foulcer Simon J; Nelson Courtney M; Quintero Maritza V; Kuberan Balagurunathan; Larkin Jonathan; Dours Zimmermann Maria T; Zimmermann Dieter R; Apte Suneel S^*

来源：JOURNAL OF BIOLOGICAL CHEMISTRY, 2014, 289(40): 27859-27873.

DOI：10.1074/jbc.M114.573287

• ADAMTS
• Chondroitin Sulfate
• Extracellular Matrix
• Glycosaminoglycan
• Metalloprotease
• Proteoglycan
• Proteolytic Enzyme
• Aggrecanase
• Blocking Antibody
• Versican

Background: The mechanisms of versican proteolysis by ADAMTS proteases are unknown. Results: The ADAMTS5 ancillary domain and specific chondroitin sulfate chains of versican are required for proteolysis. Conclusion: Docking between the ADAMTS5 ancillary domain and CS chains is a major mechanism underlying versican proteolysis. Proteolysis by ADAMTS1 has a similar requirement for GAG chains. Significance: The findings suggest strategies for blocking versican cleavage.
Proteolysis of the Glu(441)-Ala(442) bond in the glycosaminoglycan (GAG) domain of the versican-V1 variant by a disintegrin-like and metalloproteinase domain with thrombospondin type 1 motif (ADAMTS) proteases is required for proper embryo morphogenesis. However, the processing mechanism and the possibility of additional ADAMTS-cleaved processing sites are unknown. We demonstrate here that if Glu(441) is mutated, ADAMTS5 cleaves inefficiently at a proximate upstream site but normally does not cleave elsewhere within the GAG domain. Chondroitin sulfate (CS) modification of versican is a prerequisite for cleavage at the Glu(441)-Ala(442) site, as demonstrated by reduced processing of CS-deficient or chondroitinase ABC-treated versican-V1. Site-directed mutagenesis identified the N-terminal CS attachment sites Ser(507) and Ser(525) as essential for processing of the Glu(441)-Ala(442) bond by ADAMTS5. A construct including only these two GAG chains, but not downstream GAG attachment sites, was cleaved efficiently. Therefore, CS chain attachment to Ser(507) and Ser(525) is necessary and sufficient for versican proteolysis by ADAMTS5. Mutagenesis of Glu(441) and an antibody to a peptide spanning Thr(432)-Gly(445) (i.e. containing the scissile bond) reduced versican-V1 processing. ADAMTS5 lacking the C-terminal ancillary domain did not cleave versican, and an ADAMTS5 ancillary domain construct bound versican-V1 via the CS chains. We conclude that docking of ADAMTS5 with two N-terminal GAG chains of versican-V1 via its ancillary domain is required for versican processing at Glu(441)-Ala(442). V1 proteolysis by ADAMTS1 demonstrated a similar requirement for the N-terminal GAG chains and Glu(441). Therefore, versican cleavage can be inhibited substantially by mutation of Glu(441), Ser(507), and Ser(525) or by an antibody to the region of the scissile bond.

• 出版日期2014-10-3