Colorimetric detection of glutathione through tuning the oxidase-like activity of MnCO3 nanoparticles [基于碳酸锰纳米氧化酶活性调控比色检测谷胱甘肽]

作者：Zhang L.; Min Y.; Fu W.; Zhang W.; Wang Y.^*

来源：Scientia Sinica Chimica, 2022, 52(7): 1178-1186.

DOI：10.1360/SSC-2022-0069

• colorimetric detection
• glutathione
• manganese carbonate
• nanozymes
• smartphone-based assay

Nano-oxidases are a kind of nanomaterials with catalytic properties similar to natural oxidases. Regulating the catalytic activity of oxidase-like mimics is of great significance in biosensing as well as clinical diagnosis and treatment. In this article, MnCO3 nanoparticles (NPs) with oxidase-like activity were prepared by one-pot hydrothermal method, and were able to catalyze the production of reactive oxygen species from dissolved oxygen and transform colorless substrate 3,3′,5,5′-tetramethylbenzidine (TMB) into blue oxTMB with a characteristic absorption peak at 652 nm. Further studies showed that the catalytic oxidation of TMB by MnCO3 NPs could be inhibited by the addition of glutathione (GSH), and thus the production of oxTMB as well as the absorbance at 652 nm decreased, which showed a linear relationship with the concentration of GSH in a certain range. Based on this mechanism, a new colorimetric method for GSH detection based on regulating the oxidase-like activity of MnCO3 NPs was established and successfully applied to the determination of GSH in serum. With the assistance of smartphone, a portable analysis method for GSH detection was also developed with the application potential for point-of-care testing and disease diagnosis.

• 出版日期2022