An improved functional assay for rapid detection of marine toxins, saxitoxin and brevetoxin using a portable cardiomyocyte-based potential biosensor

作者：Wang, Qin; Fang, Jiaru; Cao, Duanxi; Li, Hongbo; Su, Kaiqi; Hu, Ning^*; Wang, Ping

来源：Biosensors and Bioelectronics, 2015, 72: 10-17.

DOI：10.1016/j.bios.2015.04.028

• Saxitoxin (STX)
• Brevetoxin (PbTX-2)
• Cardiomyocyte-based potential biosensor
• Multisite electrical activity of cardiomyocyte network
• Extracelluar field potential (EFP) signals

Saxitoxin (STX) and brevetoxin (PbTX-2), which are produced by marine dinoflagellates, are highly-toxic marine toxins targeting separate sites of the a subunit of voltage-dependent sodium channels (VDSCs). In this work, a portable cardiomyocyte-based potential biosensor is designed for rapid detection of STX and PbTX-2. This potential biosensor is constructed by cardiomyocyte and microelectrode array (MEA) with a label-free and real-time wireless 8-channel recording system which can dynamically monitor the multisite electrical activity of cardiomyocyte network. The recording signal parameters, spike amplitude, firing rate and 50% of spike potential duration (SPD50) extracted from extracelluar field potential (EFP) signals of the potential biosensor is analyzed to quantitatively evaluate toxicological risk of STX and PbTX-2. Firing rate of biosensor signals presents high sensitivity to STX with the detection limit of 0.35 ng/ml within 5 min. SPD50 shows high sensitivity to PbTX-2 with the detection limit of 1.55 ng/ml within 5 min. Based on the multi-parameter analysis, cardiomyocyte-based potential biosensor will be a promising tool for rapid detection of these two toxins.

• 出版日期2015-10-15
• 单位浙江大学