科研之友
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摘要
Background: Klebsiella pneumoniae-caused liver abscess (KLA) has become a health problem in Taiwan and is continually reported in other countries. Diabetes mellitus, the most common metabolic disorder, underlies half of the KLA patients in Taiwan. The clinical impact of KLA has been well-documented. Nevertheless, the molecular basis regarding how K. pneumoniae causes liver infection, particularly in diabetic individuals, remains unclear. %26lt;br%26gt;Methodology/Principle Findings: Auto-bioluminescence-expressing K. pneumoniae was inoculated into diabetic mice and age-match naive control. With the use of in vivo imaging system, translocation of the bioluminescence-expressing K. pneumoniae from intestine to extraintestinal organs, mainly the liver, was noted in 80% of the diabetic mice, whereas the same bacteria causes extraintestinal infections in only 31% of naive mice. Besides increased morbidity, the severity of hepatic tissue injury was also enhanced in the K. pneumoniae-infected diabetic mice. Upon K. pneumoniae infection, IFN-gamma production was significantly evoked in the liver. To mediate IFN-gamma signal, STAT (signal transducers and activators of transcription) 1 and 3 were activated in hepatocytes, and so was the expression of IRF (interferon regulatory factor)-1. Moreover, accumulation of neutrophils which was triggered by prolonged production of IL-1 beta and MIP-2, and significant increases in the level of active caspase 3 and phospho-eIF2 alpha, were exclusively revealed in the K. pneumoniae-infected diabetic mice. %26lt;br%26gt;Conclusion: The activation of IFN-gamma/STAT/IRF-1 signaling demonstrated by this work emphasizes the role of IFN-gamma for mediating the hepatic response to K. pneumoniae infection.
- 出版日期2013-11-6
- 单位中国医科大学
