A simple and robust high-performance liquid chromatography coupled to a diode-array detector method for the analysis of genistein in mouse tissues

作者：Tamames Tabar C; Imbuluzqueta E; Campanero M A; Horcajada P; Blanco Prieto M J^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2013, 935: 47-53.

DOI：10.1016/j.jchromb.2013.07.020

• Genistein
• HPLC
• Extraction method
• Biological matrices
• Method validation

A simple liquid-liquid extraction procedure and quantification by high-performance liquid chromatography (HPLC) method coupled to a diode-array detector (DAD) of genistein (GEN) was developed in various mouse biological matrices. 7-ethoxycoumarin was used as internal standard (IS) and peaks were optimally separated using a Kinetex C18 column (2.6 mu m. 150 mm x 2.10 mm I.D.) at 40 degrees C with an isocratic elution of mobile phase with sodium dihydrogen phosphate 0.01 M in water at pH 2.5 and methanol (55:45, v/v), at a flow rate of 0.25 mL/min. The injection volume was 10 mu L. In all cases, the range of GEN recovery was higher than 61%. The low limit of quantification (LLOQ) was 25 ng/mL. The linearity of the calibration curves was satisfactory in all cases as shown by correlation coefficients %26gt;0.996. The within-day and between-day precisions were %26lt;15% and the accuracy ranged in all cases between 90.14% and 106.05%. This method was

• 出版日期2013-9-15