Molecular characterization and identification of proteins regulated by Hfq in Neisseria meningitidis

作者：Pannekoek Yvonne^*; 't Veld Robert Huis in; Hopman Carla Th P; Langerak Ankie A J; Speijer Dave; van der Ende Arie

来源：FEMS Microbiology Letters, 2009, 294(2): 216-224.

DOI：10.1111/j.1574-6968.2009.01568.x

• Neisseria meningitidis
• Hfq
• proteomics
• riboregulation

Hfq is a highly conserved pleiotropically acting prokaryotic RNA-binding protein involved in the post-transcriptional regulation of many stress-responsive genes by small RNAs. In this study, we show that Hfq of the strictly human pathogen Neisseria meningitidis is involved in the regulation of expression of components involved in general metabolic pathways, iron metabolism and virulence. A meningococcal hfq deletion strain (H44/76 Delta hfq) is impaired in growth in nutrient-rich media and does not grow at all in nutrient-limiting medium. The growth defect was complemented by expression of hfq in trans. Using proteomics, the expression of 28 proteins was found to be significantly affected upon deletion of hfq. Of these, 20 proteins are involved in general metabolism, among them seven iron-responsive genes. Two proteins (PilE, TspA) are involved in adherence to human cells, a step crucial for the onset of disease. One of the differentially expressed proteins, GdhA, was identified as an essential virulence factor for establishment of sepsis in an animal model, studied earlier. These results show that in N. meningitidis Hfq is involved in the regulation of a variety of components contributing to the survival and establishment of meningococcal disease.

• 出版日期2009-5