Proline Derivatives in Fruits of Bergamot (Citrus bergamia Risso et Poit): Presence of N-Methyl-L-proline and 4-Hydroxy-L-Prolinebetaine

作者：Servillo Luigi^*; Giovane Alfonso; Balestrieri Maria Luisa; Cautela Domenico; Castaldo Domenico

来源：Journal of Agricultural and Food Chemistry, 2011, 59(1): 274-281.

DOI：10.1021/jf102833v

• L-proline
• 4-hydroxy-L-proline
• N-methyl-L-proline
• N,N-dimethylproline
• 4-hydroxy-L-prolinebetaine
• bergamot
• Citrus bergamia

The content of proline and various compounds deriving from its metabolism (4-hydroxy-L-proline, N-methyl-L-proline, N,N-dimethylproline, and 4-hydroxy-L-prolinebetaine) was determined in fruits and seeds of Bergamot (Citrus bergamia Risso et Poit), growing in the Calabria region (South Italy). A HPLC-ESI-tandem mass spectrometry method, which allowed rapid determination of L-proline, 4-hydroxy-L-proline, N-methyl-L-proline, N,N-dimethylproline, and 4-hydroxy-L-prolinebetaine in juice and extracts of bergamot fruit with minimum sample preparation and short analysis time (about 10 min), is presented. Proline and 4-hydroxy-L-proline levels in the samples were also determined by HPLC analysis with fluorescence detection and the results compared to those obtained with HPLC-ESI-tandem mass spectrometry. For the first time, the presence of N-methyl-L-proline and 4-hydroxy-L-prolinebetaine in the fruits of a plant of the Citrus genus is reported.

• 出版日期2011-1-12