Nanoscale Engineering of Designer Cellulosomes

作者：Gunnoo Melissabye; Cazade Pierre Andre; Galera Prat Albert; Nash Michael A; Czjzek Mirjam; Cieplak Marek; Alvarez Beatriz; Aguilar Marina; Karpol Alon; Gaub Hermann; Carrion Vazquez Mariano; Bayer Edward A; Thompson Damien

来源：Advanced Materials, 2016, 28(27): 5619-5647.

DOI：10.1002/adma.201503948

• materials modeling
• molecular dynamics calculations
• nanobiomaterials
• protein re-engineering
• renewable energy

Biocatalysts showcase the upper limit obtainable for high-speed molecular processing and transformation. Efforts to engineer functionality in synthetic nanostructured materials are guided by the increasing knowledge of evolving architectures, which enable controlled molecular motion and precise molecular recognition. The cellulosome is a biological nanomachine, which, as a fundamental component of the plant-digestion machinery from bacterial cells, has a key potential role in the successful development of environmentally-friendly processes to produce biofuels and fine chemicals from the breakdown of biomass waste. Here, the progress toward so-called designer cellulosomes, which provide an elegant alternative to enzyme cocktails for lignocellulose breakdown, is reviewed. Particular attention is paid to rational design via computational modeling coupled with nanoscale characterization and engineering tools. Remaining challenges and potential routes to industrial application are put forward.

• 出版日期2016-7-20