A novel terpenoid indole alkaloid derived from catharanthine via biotransformation by suspension-cultured cells of Catharanthus roseus

作者：He, Shuijie; Zhu, Jianhua; Zi, Jiachen^*; Zhou, Pengfei; Liang, Jincai; Yu, Rongmin

来源：Biotechnology Letters, 2015, 37(12): 2481-2487.

DOI：10.1007/s10529-015-1930-1

• Biotransformation
• Catharanthine
• Catharanthus roseus
• 3-Hydroxy-4-imino-catharanthine
• qRT-PCR
• Suspension-cultured cells

Objective Although catharanthine (1) is well known as a biosynthetic precursor of the anticancer alkaloid, vinblastine, its alternative metabolic pathways are unclear.
Results Biotransformation of 1 by suspension-cultured cells of Catharanthus roseus gave a new oxidative-cleavage product (2). The structure of 2 was determined as 3-hydroxy-4-imino-catharanthine by spectroscopic methods. Maximum conversion (9.75 %) of 2 was observed after 120 h adding 6 mg of 1/100 ml to 12-day-old suspension-cultured cells of C. roseus. Furthermore, qRT-PCR experiment was performed to reveal the effect of 1 on the expression of the genes in the biosynthetic pathway of TIA 1 up-regulated the transcript level of D4H whilst down-regulating the transcript levels of G10H, LAMT, GES, and IRS.
Conclusion A new metabolite of catharanthine, 3-hydroxy-4-imino-catharanthine, is reported.

• 出版日期2015-12
• 单位暨南大学