Uncovering the proteome response of murine neuroblastoma cells against low-dose exposure to saxitoxin

作者：Chen, Xiao; Sun, Ye; Huang, Haiyan; Liu, Wei; Hu, Panpan; Huang, Xinfeng; Zou, Fei^*; Liu, Jianjun^*

来源：Toxicology Mechanisms and Methods, 2018, 28(5): 335-344.

DOI：10.1080/15376516.2017.1411413

• Saxitoxin
• comparative proteomics
• apoptosis
• neurotoxicity

The potent neurotoxin saxitoxin produced by both marine and freshwater phytoplankton causes paralytic shellfish poisoning syndrome. The toxicity and mode of action of the acute exposure of high-dose saxitoxin have been intensively studied for decades; however, the potential risk of exposure of low-dose saxitoxin remained to be uncovered. Here we present a proteomics study of murine neuroblastoma N2A cell with low-dose saxitoxin exposure (1 nM and 10 nM, 24-h intoxication). Differential proteins were profiled by two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) coupled with matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). A total of 9 proteins, including 14-3-3 beta (1433B), alpha enolase (ENO1) and cofilin 2 (CFL2), were altered by the low-dose saxitoxin exposure. We further validated the expressions of 1433B, ENO1 and CFL2 by Western blot analysis and the enzyme-linked immunosorbent assay. These 9 proteins involve cell apoptotic pathways, cell skeleton maintenance, membrane potentials and mitochondrial functions. Modulation of these 9 proteins by low-dose saxitoxin exposure could correlate to the reports on genotoxicity and neurotoxicity induced by saxitoxin. This study also suggested other potential risks of saxitoxin.

• 出版日期2018
• 单位南方医科大学; 深圳市疾病预防控制中心