AlCl3 inhibits LPS-induced NLRP3 inflammasome activation and IL-1 beta production through suppressing NF-kappa B signaling pathway in murine peritoneal macrophages

作者：Xu, Feibo; Ji, Qiang; Zhang, Jian; Huang, Wanyue; Cao, Zheng^*; Li, Yanfei^*

来源：Chemosphere, 2018, 209: 972-980.

DOI：10.1016/j.chemosphere.2018.06.171

• Aluminium chloride
• Murine peritoneal macrophages
• NLRP3 inflammasome
• IL-1 beta generation
• NF-kappa B signaling pathway

Aluminum (Al), a common environmental pollutant, has been reported to inhibit the immune functions of macrophage. However, the mechanisms involved remain unclear. In this study, murine peritoneal macrophages were exposed to 0, 027, 0.54, and 1.08 mg/mL of aluminium chloride (AlCl3) for 24 h, and then treated with 1 mu g/mL lipopolysaccharide (LPS) for another 6 h. No addition of both AlCl3 and LPS serviced as control group. We observed that AlCl3 has cytotoxicity in murine peritoneal macrophages, showing a decrease in cell viability and an increase in lactate dehydrogenase release. Besides, AlCl3 exposure restrained the LPS-induced NLR pyrin domain containing 3 (NLRP3) inflammasome activation presented as NLRP3 expressions reduction, caspase-1 cleavage inhibition and interleukin 1 beta (IL-1 beta) maturation lessened. Meanwhile, AlCl(3 )exposure decreased LPS-induced IKK beta activity, I kappa B alpha phosphorylation, the phosphorylation and mRNA expression of NF-kappa B p65, as well the genes expression and concentration in medium supernatant of tumor necrosis factor alpha (TNF-alpha) and interleukin 6 (IL-6). The results suggested that AlCl3 inhibited the activation of NF-kappa B signaling pathway induced by LPS, which maybe one of the upstream signals involved in the inhibition of NLRP3 inflammasome activation by AlCl3. This research can provide theoretical basis for understanding the immune toxicity of Al, and deepening the cognition of Al exposure hazards to immune response.

• 出版日期2018-10
• 单位东北农业大学