An electrospun polycaprolactone-collagen membrane for the resurfacing of cartilage defects

作者：Ho Saey Tuan Barnabas; Ekaputra Andrew Krishna; Hui James Hoi; Hutmacher Dietmar Werner^*

来源：Polymer International, 2010, 59(6): 808-817.

DOI：10.1002/pi.2792

• electrospun mesh
• polycaprolactone
• collagen and periosteal substitute

A polycaprolactone (PCL)-collagen electrospun mesh is proposed as a novel alternative to the conventional periosteal graft in autologous chondrocyte implantation. This is the first known attempt in designing a cartilage resurfacing membrane using a mechanically resilient PCL mesh with a weight-average molecular weight of 139300 that is enhanced with bioactive collagen. PCL-collagen 10, 20 and 40% electrospun meshes (Coll-10, Coll-20 and Coll-40) were evaluated and it was discovered that the retention of surface collagen could only be achieved in Coll-20 and Coll-40. Furthermore Coll-20 was stiffer and stronger than Coll-40 and it satisfied the mechanical demands at the cartilage implant site. When seeded with mesenchymal stem cells (MSCs), the cells adhered on the surface of the Coll-20 mesh and they remained viable over a period of 28 days; however, they were unable to infiltrate through the dense meshwork. Cell compatibility was also noted in the chondrogenic environment as the MSCs differentiated into chondrocytes with the expression of Sox9, aggrecan and collagen II. More importantly, the mesh did not induce a hypertrophic response from the cells. The current findings support the use of Coll-20 as a cartilage patch, and future implantation studies are anticipated.

• 出版日期2010-6