Aminoguanidine attenuates hypertension, whereas 7-nitroindazole exacerbates kidney damage in spontaneously hypertensive rats: The role of nitric oxide

作者：Huang Chien Fu; Hsu Chien Ning; Chien Shao Ju; Lin Ying Jui; Huang Li Tung; Tain You Lin^*

来源：European Journal of Pharmacology, 2013, 699(1-3): 233-240.

DOI：10.1016/j.ejphar.2012.11.034

• Asymmetric dimethylarginine
• Hypertension
• Kidney injury
• Neuronal nitric oxide synthase
• Nitric oxide

Nitric oxide (NO) deficiency contributes to hypertension and end-organ damage. Three nitric oxide synthase (NOS) isoforms have been identified: neuronal NOS (nNOS), inducible NOS (iNOS), and endothelial NOS (eNOS). Whether selective nNOS or iNOS inhibition exacerbates kidney damage in spontaneously hypertensive rats (SHRs) remains unclear. Seven-week-old SHRs were randomly assigned to 4 groups (n=8 for each group): group 1, SHRs receiving no treatment; group 2 (SHR+7-NI), SHRs given7-nitroindazole (7-NI, nNOS inhibitor) in their drinking water (10 mg/kg/day); group 3 (SHR+salt), SHRs given 1% NaCl; and group 4 (SHR+AG), SHRs given0.1% aminoguanidine (AG; iNOS inhibitor) in drinking water. The mean arterial pressure of SHRs treated with salt was significantly elevated compared with untreated controls. While AG caused a decrease of mean arterial pressure at 8 and 12 weeks of age in SHRs, both 7-NI and salt exacerbated kidney injury. In addition, AG significantly increased L-arginine levels and the L-arginine-to-asymmetric dimethylarginine (ADMA) ratio in the kidney. Salt treatment decreased renal nNOS-alpha protein levels and reduced dimethylarginine dimethylaminohydrolase (DDAH) activity. Salt and AG treatment increased nNOS-beta and L-citrulline levels in SHR kidneys. AG attenuates hypertension development by upregulation of L-citrulline-to-L-arginine conversion and an increase in the L-arginine-to-ADMA ratio in SHR kidneys. 7-NI impairs renal function but has no effect on blood pressure, suggesting reno-protective role for the nNOS. Salt exacerbates kidney damage mainly through decreasing renal nNOS-alpha protein levels and DDAH activity. Our findings highlight the protective role of the nNOS/NO pathway in the development of kidney damage in SHRs.

• 出版日期2013-1-15
• 单位长春大学