HYBRIDIZATION AND MOLECULAR CHARACTERIZATION OF F-1 ALLIUM CEPA X ALLIUM ROYLEI PLANTS

作者：Chuda Alicja^*; Adamus Adela

来源：Acta Biologica Cracoviensia Series Botanica, 2012, 54(2): 25-31.

DOI：10.2478/v10182-012-0016-9

• Allium cepa
• A. roylei
• embryo rescue
• genomic in situ hybridization (GISH)
• interspecific hybrids
• molecular markers

We made interspecific crosses to facilitate the introgression of desirable traits of Allium roylei into the Allium cepa genome. After hand-pollination, 906 interspecific F-1 Allium cepa x A. roylei plants were obtained by in vitro culture via embryo rescue. Nuclear DNA analysis showed that 97.6% of the regenerants were interspecific F-1 Allium cepa x A. roylei hybrids. Genomic in situ hybridization (GISH) showed that each hybrid had 16 chromosomes, eight of which were identified as A. cepa and eight as A. roylei chromosomes.

• 出版日期2012