Direct electrochemical evaluation of plasma membrane cholesterol in live mammalian cells

作者:Jiang Dechen; Devadoss Anando; Palencsar M Simona; Fang Danjun; White Nicole M; Kelley Thomas J; Smith Jonathan D; Burgess James D*
来源:Journal of the American Chemical Society, 2007, 129(37): 11352-+.
DOI:10.1021/ja074373c

摘要

Cholesterol is a tightly regulated structural component of the cell plasma membrane. Dysfunctional intracellular cholesterol transport machinery causes disease states with altered plasma membrane cholesterol. This Communication reports microelectrode evaluation of plasma membrane cholesterol of single cells at physiological temperature. Electrochemical data indicate that transport of intracellular cholesterol to the plasma membrane is active in an atherosclerotic macrophage model.

  • 出版日期2007-9-19