Analgesic and anti-inflammatory activities of epicatechin gallate from Bauhinia hookeri

作者：Al Sayed Eman^*; Abdel Daim Mohamed M

来源：Drug Development Research, 2018, 79(4): 157-164.

DOI：10.1002/ddr.21430

• analgesic
• anti-inflammatory
• cytokines
• epigallocatehin gallate
• tea catechins

The acetic acid-induced writhing and hot plate models in mice were utilized to determine the analgesic effect of epicatechin gallate (ECG) isolated from Bauhinia hookeri. The anti-inflammatory activity of ECG was determined using carrageenan-induced paw edema model. The pro-inflammatory mediators (PGE(2), TNF-, IL-1, and IL-6) were estimated in the plasma of different treatment groups. ECG was tested at doses of 100, 200, and 400 mg/kg p.o. and diclofenac sodium was used as a standard drug (100 mg/kg) in all experiments. ECG significantly (p<.001) suppressed the writhing response in mice. The inhibition percentages were 32, 52, and 62%, at the tested doses of ECG, respectively as compared to the positive control group receiving acetic acid only. Furthermore, ECG significantly (p<.001) increased the reaction time in hot plate model. The maximum analgesic effect was evident after 120 min. ECG demonstrated a significant anti-inflammatory activity as evidenced by the inhibition of carrageenan-induced paw edema (46, 50, and 58%, at the tested doses, respectively). This effect was persistent all over the experimental period. ECG produced a significant (p<.001) reduction in plasma PGE(2) (by 27, 38, and 50%), TNF- (15, 33, and 41%), IL-1 (17, 25, and 33%), and IL-6 (22, 32, and 43%), at the tested doses, respectively. This study supports the use of ECG as both analgesic and anti-inflammatory agent.

• 出版日期2018-6