科研之友
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摘要
Variation at the Xgwm261 locus was studied in 92 Indian bread wheat varieties. The locus is of interest because its 192 bp allele is reported to be linked to semi-dwarfing gene Rht8. There were five allelic variants based on size. Occurrence of 165, 192 and 174 bp alleles was 44.5, 39.1 and 10.8%, respectively. Sequencing showed six new alleles. Cvs. C306 and PBW516 showed 192 bp alleles which were different from each other, different from other varieties and also from reported sequence. A 200 bp band in four varieties was unique and different in CT repeats and sequence motif next to the repeat region. Cv. NI179 showed new 198 bp allele. The varieties were also analysed with allele specific primers for Norin10 genes. Among semi-dwarf varieties, frequencies of RhtB1b and RhtD1b were 48.5 and 45.7%, respectively. The relation between alleles of Xgwm261 and agronomic traits was studied by growing plants at two locations. The 192 bp allele did not show association with grain yield or its components at Trombay location. Differences in plant height among the 192, 165 and 174 bp allele carriers were not significant at both the locations indicating that the 192 bp allele in Indian varieties was neutral with respect to plant height. Frequency of 165 bp allele was higher in varieties recommended for warmer zones and late sown conditions.
- 出版日期2012-3
