Elucidation of the Mass Fragmentation Pathways of Tomatidine and beta 1-Hydroxytomatine using Orbitrap Mass Spectrometry

作者：Caprioli Giovanni^*; Cahill Michael; Logrippo Serena; James Kevin

来源：Natural Product Communications, 2015, 10(4): 575-576.

DOI：10.1177/1934578x1501000409

• Tomato glycoalkaloids
• Structural elucidation
• Orbitrap MS
• Tomatidine
• beta-Hydroxytomatine

Tomatoes, members of the Solanaceae plant family, produce biologically active secondary metabolites, including glycoalkaloids, which may have both adverse and beneficial biological effects. Using the linear ion trap (LIT) mass spectrometry, multi-stage collision induced dissociation (CID) experiments (MSII) were performed to elucidate characteristic fragmentation pathways of the glycoalkaloid, tomatidine and of beta(1)-hydroxytomatine. High resolution with high accuracy mass analysis using an Orbitrap fourier transform MS with higher-energy collisional induced dissociation (HCD) was used to produce mass spectra data across a wide spectral range for confirmation of proposed ion structures and formulae.

• 出版日期2015-4