Nuclear factor-kappa B as a promising target for selenium chemoprevention in rat hepatocarcinogenesis

作者：Alwahaibi Nasar Y^*; Budin Siti B; Mohamed Jamaludin; Alhamdani Aisha

来源：Journal of Gastroenterology and Hepatology, 2010, 25(4): 786-791.

DOI：10.1111/j.1440-1746.2009.06160.x

• cyclin D1
• hepatocarcinogenesis
• nuclear factor-kappa B
• selenium
• transforming growth factor-alpha

Background and Aims:
Selenium's molecular mechanism for cancer chemoprevention remains unknown. We aimed to study the gene expression of nuclear factor-kappa B (NF-kappa B), tumor growth factor-alpha (TGF-alpha) and cyclin D1 and the effects of sodium selenite using preventive and therapeutic approaches in chemically-induced hepatocarcinogenesis in rats.
Methods:
Rats were divided randomly into six groups: negative control, positive control (diethyl nitrosamine [DEN] + 2-acetylaminofluorene [2-AAF]), preventive group, preventive control (respective control for preventive group), therapeutic group and therapeutic control (respective control for therapeutic group). The relative gene expression of NF-kappa B, TGF-alpha and cyclin D1 in liver tissues were measured using real-time polymerase chain reaction.
Results:
The findings showed that the gene expression of NF-kappa B in the preventive group and its respective control was significantly lower (P < 0.05) when compared with both the negative and positive controls. However, the expression of NF-kappa B in the positive controls and therapeutic group was significantly higher (P < 0.05) when compared with the negative controls. The expression of TGF-alpha and cyclin D1 was insignificant in all groups.
Conclusion:
The inhibition of the NF-kappa B pathway in the initiation phase of hepatocarcinogenesis could be a promising target for selenium chemoprevention. However, further studies are required.

• 出版日期2010-4