Narrow-leafed lupin (Lupinus angustifolius L.) β-conglutin proteins modulate the insulin signaling pathway as potential type 2 diabetes treatment and inflammatory-related disease amelioration

作者：Lima-Cabello, Elena; Alche, Victor; Foley, Rhonda C.; Andrikopoulos, Sofianos; Morahan, Grant; Singh, Karam B.; Alche, Juan D.; Jimenez-Lopez, Jose C.^*

来源：Molecular Nutrition & Food Research, 2017, 61(5): 1600819.

DOI：10.1002/mnfr.201600819

• Anti-inflammatory
• Antioxidant
• GLUT-4
• IL-1 beta
• Legumes
• PI3-kinase
• Sweet lupins
• Type 2 diabetes
• Vicilin

Scope: We have investigated the potential use of beta-conglutin protein isoforms from narrow-leafed lupin (Lupinus angustifolius L.) as a diabetes treatment. @@@ Methods and results: We produced purified recombinant beta 1-, beta 2-, beta 3-, beta 4-, and beta 6-conglutinproteins and showed that beta 1, beta 3, and beta 6 could bind to insulin. To assess beta-conglutin proteins modulatory effect on insulin activation meditated kinases, whole blood and peripheral blood mononuclear cell cultures from type 2 diabetes (T2D) and healthy control subjects (C) were incubated with conglutin proteins. The treatment of peripheral blood mononuclear cells from T2D patients with beta 1, beta 3, and beta 6 proteins increased up to threefold mRNA and protein levels of genes important in insulin signaling pathways, namely insulin receptor substrate 1/p85/AKT/glucose transporter type 4. This was accompanied by a comparable fold-change decrease in the mRNA expression level of pro-inflammatory genes (iNOS and IL-1 beta) and proteins compared to healthy controls. The beta 2 and beta 4 isoforms had no effect on the insulin signaling pathway. However, these beta-conglutin proteins elicited pro-inflammatory effects since levels of mRNA and proteins of inducible nitric oxide synthase and IL 1 beta were increased. @@@ Conclusion: Our results raise the possibility of using these particular beta-conglutin proteins in the prevention and treatment of diabetes, as well as their potential as anti-inflammatory molecules.

• 出版日期2017-5
• 单位河北医科大学