Nutrient mixture including vitamin C, L-lysine, L-proline, and epigallocatechin is ineffective against tumor growth and metastasis in a syngeneic neuroblastoma model

作者：Lode Holger N; Huebener Nicole; Strand**y Anne; Gaedicke Gerhard^*

来源：Pediatric Blood and Cancer, 2008, 50(2): 284-288.

DOI：10.1002/pbc.21172

• ascorbic acid
• epigallocatechin
• L-lysine
• neuroblastoma

Background. The replacement of established evidence-based cancer therapy protocols (mainstream therapy) by unevaluated complementary and alternative medicine (CAM) is a challenge in pediatric oncology. We tested the hypothesis that oral application of L-lysine and ascorbic acid (Lysin C Drink (R) ) in combination with epigallocatechin-gal late (EGCG) and amino-acids (Epican forte (R)) is effective in a preclinical model of neuroblastoma. Methods. Primary tumors and spontaneous metastases were induced in A/J mice by injection of NXS2 neuroblastoma cells. Mice were treated by daily oral gavage With L-lysine and ascorbic acid (Lysin C Drink (R)) (equivalent to 150 mg ascorbic acid/day/mouse) (treatment A) or with EGCG Plus ascorbic- and amino-acids (Epican forte (R)) (9.2 mg/mouse) (treatment B). Treatment A was started in the prophylactic setting (7 days before tumor cell injection) as well as in the therapeutic setting (1 day after tumor cell inoculation). Finally, treatment B was evaluated alone and in combination with treatment A in the therapeutic setting. The effect on primary tumor growth and the development of spontaneous liver metastases was evaluated. Results. L-lysine and ascorbic acid (Lysin C Drink (R)) and EGCG plus ascorbic- and amino-acids (Epican forte (R)) are ineffective in reduction of primary tumor growth and prevention of spontaneous liver metastases in this model. Conclusions. Neither a formal clinical development nor the use of these substances can be recommended for neuroblastoma.

• 出版日期2008-2